不同生长时期梅花鹿鹿茸转录组分析

doi:10.11843/j.issn.0366-6964.2017.02.006

Abstract

Abstract:

The objective of this study was to find the transcriptome differences of sika deer antlers at different stages, and enrich the sika deer antler transcriptome data. The sika deer antlers aged 10, 20, 28, 44 and 66 d were selected as experimental material，then the transcriptome libraries of antlers were constructed by using Illumina HiSeq^TM 2500 platform, and the transcriptome data were analyzed by bioinformatics methods, such as sequencing assess and gene annotation. The results showed that a total of 375 657 contigs with an average length of 688 bp were generated, from which 329 946 unigenes with an average length of 534 bp were defined. 90.07% of the 297 198 unigenes were annotated in public databases of Nr，Nt，Pfam，KOG/COG，Swiss-prot，KEGG and GO. Through annotation classification, a total of 39 674(12.02%) genes were divided into 41 GO function categories at level 2, and 17 732 (5.37%) genes were annotated in 26 KOG categories. Comparative analysis of differentially expressed genes in sika deer antler at 5 different stages, and 509 differentially expressed genes were screened, from which 407 genes were annotated in GO database including signal transduction, oxidation-reduction process, regulation of transcription, proteolysis, and so on. And the expression of PER1 and EGR1 (transcription regulation gene) increased with the growth of antlers, on the contrary, the expression of GAS1 decreased. It indicates that PER1, EGR1, GAS1 may have important role in regulation of antler growth. The transcriptome study of the antler tissue in the different growth period revealed the number of differentially expressed genes by using high-throughput sequencing technology, obtained the function of differentially expressed genes.

CLC Number:

S825
S813.1

ZHANG Ran-ran, LIU Hua-miao, WANG Lei, XING Xiu-mei, SU Wei-lin, GAO Bing. Transcriptome Analysis of Sika Deer Antlers at Different Stages[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(2): 235-242.

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Transcriptome Analysis of Sika Deer Antlers at Different Stages

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